Composite

Part:BBa_J44003:Design

Designed by: Todd Eckdahl   Group: iGEM06_Missouri   (2006-08-16)


HixC-pBad-HixC-RBS-TetF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 159
    Illegal NheI site found at 371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 99
    Illegal BamHI site found at 517
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 543
    Illegal NgoMIV site found at 911
    Illegal NgoMIV site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pBAD is able to be flipped by the Hin recombinase.


Source

HixC (J44000), pBAD (I13453), HixC (J44000), RBS (B0030), TetF (J31007)

References